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This information has been used to develop two different microarray platforms, based on cDNA and short oligo sequences [ 12, 17].
Over the past years, many microarray platforms, based on different technologies, have been developed by commercial and academic institutions.
Microarray platforms based on either oligonucleotides or purified amplification products have been utilised in parallel to produce large amounts of data.
What is lacking in these studies is a "gold standard" data set that allows an evaluation of different microarray platforms based on a common "ground truth".
While the main focus of the study was to directly compare the data generated from the two microarray platforms based on detected transcripts and pathways and for this a single model cell line would be appropriate, however, a secondary aim was to assess the biological relevance of the colorectal transcriptome and compare this to a generic genomic approach.
It is well known that meaningful comparative expression analyses using microarray platforms based solely on EST collections can be difficult because of the frequent and confounding presence of multiple splice forms, paralogs and orthologs, as well as, in the case of polyploids, homoeologs with near-identical sequence [ 31].
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We used microarray platform based on a different songbird species (zebra finch) to measure gene expression in the song sparrow.
We have compared miRNA profiles of early versus senescent MSCM1 passages using a microarray platform based on locked nucleic acids (miCHIP) [29].
A microarray platform based on this Ar.
However, this species lacks a platform for global gene expression profiling, so we designed a first generation high-density oligonucleotide microarray platform based on channel catfish EST sequences.
We designed microarray probes for two-colour Agilent microarray platform based on the predicted gene models of the reference genome sequence.
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