In particular, differences in the structure and sensitivity/reproducibility of microarray platforms, and in the variety of tools used to analyze microarray data, strongly influence experimental outcome.
She's also created artificial bone materials, targets for tuberculosis therapy, cell microarray platforms, and a cell nanoinjector, a device used to inject molecules into living cells.
The probe annotations for all microarray platforms and qRT-PCR were provided by the manufacturer.
As newer microarray platforms and approaches have permitted complete or near complete genome wide inquiry, this problem may be resolved.
A total of 15,809 expression features were available from 25 studies, utilizing 10 different microarray platforms, and comprising a total of 2,744 patient samples (Table 1).
This apparently was due to the fact that a few genes with strong prognostic power were not available on all microarray platforms and thus were not retained in the merged data sets.
The partially non-overlapping results reported in above-cited studies may be attributed either to the use of different microarray platforms and analysis methods or to the use of different cell lines and growth conditions.
Representative scatter plots of microarray platforms and the Taqman system are displayed in Figure 2A (scatter plots for all possible combinations between three replicates were shown in Figure S4).
We used Partek Software analyses to integrate the three microarray platforms and to identify subsets of genes with methylation/expression patterns that were either dependent (or independent) of gene copy number.
To compare measurements across a range of amplification methods, several microarray platforms and other quantification methods, such as qPCR and massive parallel sequencing, we used a uniform method to analyze performance.
Pathway analysis of the combined Affymetrix and Illumina datasets demonstrates a high degree of consistency between microarray platforms and between NRO and total RNA at the geneset level (Figure 3b).
