Sentence examples for microarray platforms Additional from inspiring English sources
In total 8,125 orthologous genes were represented on both microarray platforms (Additional file 5).
This indicated that these rejection-associated gene expression signatures were consistent across the 2 microarray platforms (Additional file 9, C and D).
All experiments data on pig response to diverse pathogens, time period of observation, challenge system (in vivo/ in vitro), pig tissues/cells and microarray platforms (Additional file 1: Table S1), were considered for this study.
Quantitative reverse transcription PCR (qRT-PCR) was used to validate the results of the different microarray platforms [see Additional file 2].
Then, we describe the transfer of the optimal signature genes from the microarray platform, plus additional prognostic genes identified, to a PCR platform for easier clinical implementation of the molecular classifier.
First, all publically available datasets from immune response experiments using many different microarray platforms and pathogens (Additional file 1: Table S1), including the ones for the response to PRRSV, were considered for the first global meta-analysis.
Two algorithms were subsequently used to construct CNVR maps, which were also validated by processing subsamples with additional microarray platforms (Illumina 1M-Duo BeadChip, Nimblegen 385K aCGH array) and by comparing our data with publicly available information.
Technological improvements to achieve a higher resolution have resulted in the generation of additional microarray platforms encompassing larger numbers of shorter DNA targets (oligonucleotides).
Furthermore, the prognostic predictability of CMTC agreed very well with all 14 prognostic gene signatures that were developed independently using different commercial microarray platforms (Table S3 in Additional file 5).
Steroid biosynthesis was also the most down-regulated pathway in liver-conditional knockout mutants on probucol therapy (Supporting information File 5), a result technically validated on three additional microarray platforms (Zhang et al, 2010).
An independent validation cohort consisting of publicly available gene expression array data from 2,487 breast cancers was compiled from different published original reference data sets that used Agilent and Affymetrix microarray platforms (Table S2 in Additional file 2).
