An associated microarray platform derived from this work will enable the investigation of functional genomics of circadian rhythmicity, photoperiodic time measurement, and diapause along a photic and seasonal geographic gradient.
In the multifaceted experiments, all six consortium centers used two common platforms: an oligo microarray manufactured at one of the centers and the commercial Agilent mouse microarray platform, developed by TRC investigators working collaboratively with Agilent and the NCT microarray resource contractor, Paradigm Genetics.
The robustness and reliability of the mouse NIA-15K cDNA microarray platform has been demonstrated in our previous work [ 11, 12] and by others [ 13- 15].
We previously developed a custom miRNA microarray platform, and here we describe the protocol we used to work with it including the oligo design strategy, the microarray printing protocol, the target-probe hybridization and the signal detection.
In this work we successfully designed and tested a novel microarray platform for Chlamydomonas reinhardtii.
In previous work we demonstrated the ability of pilot versions of the peptide microarray platform to identify novel ligands for a number of specific protein modules [11], [13] [15].
Previous work has shown that the experimental FDR for a porcine brain microarray platform followed statistical expectations without the addition of a fold cut-off and that additional selection criteria could be used to virtually eliminate false positives [ 29].
More complete validations of antisense transcripts detected by our microarray platform were conducted by RT-PCR, Northern analysis, and in situ hybridization in our previous work [ 11].
In this work gene expression was assayed in four patients with psoriasis using HUG95A Affymetrix microarray platform.
While some empirical work has been carried out on probe/target identity (PTI) and data analysis using the microarray platform, the focus has largely been on optimization of species detection and/or identification in complex samples [ 21- 23].
