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Finally, inhibitor-treated samples were studied using a 17 K gene expression microarray platform, whereas siRNA-treated samples were hybridized on a 44 K microarray platform causing slight differences in the gene content of these two microarray platforms.
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These experiments mainly used microarray platforms whereas within the last 5 years mainly next generation sequencing approaches (e.g. RNA-Seq) were carried out and broadened our knowledge about gene expression networks under stress conditions.
The microarray platform was designed according to the 2006 version genome annotation, whereas the RNA-Seq data were analysed using the 2008 version genome annotation.
Human microarray platform, GPL1528; human HCC microarray data, GSE1898; mouse microarray platform, GPL1529; mouse HCC microarray data, GSE1897.
A robust small-molecule microarray platform for screening cell lysates.
We generated the data from each mouse model using the same microarray platform and reference RNA.
Shippy, R. et al. Using rna sample titrations to assess microarray platform performance and normalization techniques.
Multiplexing in the microarray platform was supported by a medium throughput protein expression system.
When genes were represented more than once in the microarray platform, we used the averaged expression ratios.
Then select the microarray platform and specific experiment to search against and the display format.
The accumulated transcribed sequences could be directly used to develop microarray platform.
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