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Using a custom microarray platform, we examined expression of 366 genes of interest in peel pericarp and endocarp during three developmental stages of Washington Navel orange fruit (Citrus sinensis L. Osbeck).
It remains to be determined if nonadditive expression detected for other genes in our microarray analysis might be explained by specific homoeologous rearrangements in the lines, since the microarray platform we employed was unable to distinguish between related transcripts.
To compare the similarity of the log ratio for each microRNA between each microarray platform, we determined the slope and intercept of the orthogonal regression between pairs of the log ratio in each microarray platform.
To facilitate a direct comparison between these profiles using the same microarray platform, we prepared A673 Ewing's sarcoma cells consisting of "EWS/FLI-expressed" (luc-RNAi and EF-2-RNAi/EWS/FLI cDNA rescue cells) or "EWS/FLI-knockdown" (EF-2-RNAi and EF-2-RNAi/ empty-vector" EF-2-RNAi/ empty-vectoralyzEF-2-RNAi/ empty-vector133plus2 microarrays.
Using the exon microarray platform, we describe a novel approach to study alternative promoter activity using hybridization probes that anneal to first exons of genes, which enables us to evaluate the exclusive activity of the alternative promoters located upstream of these exons.
To assess signal repeatability and reproducibility of each microarray platform, we utilized the methods that the MAQC Project used [6], such as calculating the Spearman's correlation coefficient (Rs), and the coefficient of variation (CV) of the signal or Cy3/Cy5 values for one or two color method, respectively.
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In order to better understand the sensitivity, dynamic range, and reproducibility of three common DNA microarray platforms, we compared two human postmortem samples on cDNA microarrays with dual-fluorescence, oligonucleotide GeneChips® (Affymetrix), and single-color gel matrix deposited CodeLink® oligonucleotide arrays.
To compare the microRNA profile between two different microarray platforms, we used all overlapped microRNAs available in both microarray platforms.
As is the case for many microarray platforms, we found that proper oligonucleotide design is critical.
For Rosetta microarray platforms, we used mapping between probes and NCBI Entrez genes available at Gene Expression Omnibus (GEO) database.
Considering differences in microarray platforms, we selected common genes between the Agilent Whole Human Genome Oligo Microarray and Affymetrix Human Genome U133 Plus 2.0 Array, which was the platform in an external dataset (GSE9891) [20].
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