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Gene expression profiling the 44 K microarray platform was performed in root samples at 1, 5, and 10 days after –P and ++P stress treatments.
Initially, a microarray platform was set up using an unrelated in-house antigen, PUUV recombinant nucleocapsid protein, to optimize the protocol for the detection of antibodies.
The NimbleGen S. solfataricus (P2) microarray platform was used to assess the organism's transcriptional response to 30 µM H2O2.
Each microarray platform was deployed at three independent test sites and five replicates were assayed at each site.
A microarray platform was used to establish IFN-related gene expression profiles in the specimens collected from the 87 CH and 5 NL samples (Table 1).
The corresponding correlation coefficients (R2) were >0.98, demonstrating that inter- and intra-chip variation of the microarray platform was quite low.
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The design, construction and application of a Pinus microarray platform are described.
The microarray platform is a powerful tool for conducting large‐scale, high‐throughput gene expression experiments.
Besides the DNA-based array assay, the microarray platform is also being rapidly expanded to include the analysis of other biomolecules such as proteins and carbohydrates.
The microarray platform is Affymetrix ATH1 GeneChip.
Patient demographic data, clinical characteristics, analysis group, and microarray platform are summarized in Table 1.
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