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In this study, we developed an oligo microarray platform using all available I. multifiliis expressed sequence tag (EST) information as well as probes designed through comparative genomics to other protozoa.
The expression patterns of these genes were validated in a different microarray platform using material from three different hematopoietic progenitor and stem cell experiments.
Affymetrix GeneChips are a popular oligonucleotide microarray platform, using probe sets formed by 11-20 pairs of 25 mer probes.
The majority of the studies reported to date have utilised the DNA microarray platform using both oligonucleotide and cDNA formats.
However, dataset-specific bias precludes meta-analysis at the raw transcript level, even when the RNA is from comparable sources and has been processed on the same microarray platform using similar protocols.
To find the miRNAs that may be involved in dark-induced leaf senescence, we identified miRNAs by microarray platform using Arabidopsis thaliana leaves from both whole darkened plants (DPs) and individually darkened leaves (IDLs).
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The sensitivity of the microarray platform used here has allowed us to monitor miRNA from a small volume of serum and to classify sera as either from normal donors or from cancer patients.
This discrepancy arises due to the difference in microarray platform used.
The next level of clustering was defined by the microarray platform used for the studies.
The microarray platform used was a printed 19 K oligonucleotide set (18,861 oligonucleotides representing 18,664 unique sequences) from Sigma-Genosys, Cambridge, UK).
We selected to also analyze the NCBI GEO GSE593 ULM profiling along with the data from this study due to the same sample size, patient age and the microarray platform used [21].
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