Sentence examples for microarray platform see from inspiring English sources

Data normalization: a global normalization was performed across all data from a given microarray platform (see Methods section).

Each of the 16 pools was then independently hybridized to an Amby_001 custom Affymetrix GeneChip (for a more detailed description of the microarray platform see [ 31] and [ 32]).

Each dataset is composed of multiple technical replicates for several distinct microarray platforms (see Supplementary Table S1).

The analysis was performed including the expression values of 9.963 genes identified as orthologous between the two species and represented on the respective microarray platforms (see Methods).

To convert from a source to a target microarray platform, we chose to model each individual sequence tag in the target platform as a linear combination of all sequence tags from the source platform (see Fig. 1 and Supplementary Methods).

RNAseq is a more powerful technique than microarrays, and relies on a different experimental/statistical approach (for a comparison between microarray and RNAseq platforms see Guo et al. [ 53]), which may hinder comparisons among studies.

The 18 mechanism scores were computed from between 1110 and 1387 total unique genes, depending on the microarray platform used in each study (see Additional files 2 and 3), with significant overlaps between the gene sets for each mechanism (Fig.  3).

In general, RT-qPCR results confirmed the regulation trend seen in the microarray platform for all the 11 genes analyzed with very similar values using either GAPDH or ACTB for normalization.

For comparison, using the Febit microarray platform, 653 differentially expressed genes were identified (fold change >1.5) (see Additional file 1: Table S3, Table S4, and Table S5).

For each microarray platform, gene annotation was performed with the MADGene tool [ 70] (see also part 6 of the Additional File 3 for more details on this database).

Microarray analysis was performed using the GeneChip Human Genome U133 Plus 2.0 oligonucleotide microarrays (Affymetrix) for gene expression profiling, and a custom in-house manufactured miRNA microarray platform (A-MEXP-620 version 3.0) to profile the expression of miRNAs (see additional files 1 and 2: Tables S1 and S2 and materials and methods for accession number).