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This miRNA microarray platform generates reproducible data and is recommended for the study of changes in miRNA expression [ 25].
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These data indicate that the four microarray platforms generated highly reproducible data that are in-line with previous cross-platform papers.
These data indicate that miRNA microarray platforms generated highly reproducible data and can be recommended for the study of changes in miRNA expression.
Since the microarray platform only generates relative expression values, the correlation between the RNA-seq data and the microarray data was calculated using the log2 value of the ratio between A431 and U251, which in the RNA-seq case yields one value per Ensembl-gene.
These AFAS probes were spotted onto microarray slides and used in our microarray platform to generate expression data for the analysis of antisense transcripts.
Details of the array construction are described elsewhere (Moreira LM, Laia ML, de Souza RF, Zaini PA, da Silva ACR, Ferro JA, da Silva AM: Development and validation of a Xanthomonas citri subsp. citri DNA microarray platform (XACarray) generated from the shotgun libraries previously used in the sequencing of this bacterial genome, submitted).
Also the Nimblegen platform generates these probes using photolithography with masks technology instead of the inkjet method for fabrication employed by the Agilent microarrays.
Biological networks captured by the different microarray platforms were generated using IPA and scored based on the relationship between the total number of genes in the specific network and the total number of genes identified by the microarray analysis.
Our laboratory used two microarray platforms to generate global mRNA expression profiles for both aneurysmal and non-aneurysmal human abdominal aorta.
Since 2004, the vast majority (29/32) of technical papers comparing microarray platforms have generated results that show a moderate to high degree of correlation among the technologies.
The present study utilized two microarray platforms to generate global mRNA expression profiles for both aneurysmal and non-aneurysmal abdominal aorta.
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