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Allele-specific oligonucleotide hybridization (ASH) on the massively parallel DNA microarray platform encompasses a simple and powerful method for high-throughput genotyping.
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Both reference and test aRNA (6 µg of each sample) were directly labeled using ULS aRNA Fluorescent Labeling kit (Kreatech, Salt Lake City, UT) and co-hybridized to a custom-made 36K oligo-based microarray platform encompassing the whole human genome.
Technological improvements to achieve a higher resolution have resulted in the generation of additional microarray platforms encompassing larger numbers of shorter DNA targets (oligonucleotides).
Then select the microarray platform and specific experiment to search against and the display format.
The accumulated transcribed sequences could be directly used to develop microarray platform.
The design, construction and application of a Pinus microarray platform are described.
After entering a list of genes or microarray element IDs into the ROAD, the user can then select the microarray platform and specific experiment to search against (Figure1a).
The usefulness and accuracy of an oligonucleotide microarray platform relies on careful design of the oligo probes.
This indicates that 95% of the transcripts in the 44 K microarray platform could be validated by mRNA-Seq.
However, no microarray platform has been experimentally optimized for studying the transcriptome of field isolates.
Here a novel electrochemical microarray platform towards high-throughput detection is presented.
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