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To this end, we conducted a comprehensive genetic study of fatty acid profiles in IBD using iCHIP, a custom microarray platform designed for deep sequencing of immune-mediated disease associated loci.
We used a microarray platform designed for formalin-fixed, paraffin-embedded (FFPE) tissue specimens, the Affymetrix OncoScan FFPE Express 330K Molecular Inversion Probe (MIP) array, to explore the correlation between genomic imbalances detected by microarray and FOXL2 mutation status detected by pyrosequencing in a series of 21 archived AGCTs.
In this scenario, a high-density microarray platform designed for aCGH would greatly improve the efficiency of detection and estimation of SVs.
Using amplified messenger RNA and a custom microarray platform designed to interrogate expression for 40,430 genes, we determined global patterns of expression during fibre development.
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It suggests that a standard set of microRNAs would be needed to compare the performances between microRNA microarray platforms designed according to different miRBase versions.
The microarray platform design is available at array express accession number A-MEXP-2065 A-MEXP-2065 A-MEXP-2065
Our results may be useful for improving future versions of microarray platform design, especially those which involve the single-base mismatches such as the Affymetrix GeneChip.
The comprehensive assessment of the effects of single-base mismatches on microarrays provided in this report can be useful for improving future versions of microarray platform design and the corresponding data analysis algorithms.
A custom whole-genome gene expression high-definition oligo microarray platform, (design ID 021695, catalogue No. G2514F), was created and made publicly available in the Gene Expression Omnibus (GEO) database (GPL14111).
It is worth mentioning that ITGB3, ITGB6 and ITGB8, elements that interact with ITGAV, are present in the microarray platform design, but were excluded from analysis in the filtering steps (see Methods section).
A custom microarray for B. cenocepacia J2315 was used, [ 10] however, the design was updated with each probes printed four times in a randomised distribution using the Agilent SurePrint 4 × 44K microarray platform (design AMADID 017397; ArrayExpress http://www.ebi.ac.uk/arrayexpress accession number A-MEXP-1613 A-MEXP-1613 A-MEXP-1613
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