Sentence examples for microarray platform confirmed from inspiring English sources
The 70-mer oligonucleotide microarray platform confirmed the directionality of the expression differences between parental inbred genotypes for the vast majority of the genes identified by Affymetrix.
The fold-changes found by qRT-PCR were slightly higher than those found by any of the microarray platforms, confirming previous observations that ratios tend to be compressed in microarray experiments [ 21, 23, 35].
As expected the two genes found as DEGs by all five microarray platforms were confirmed to display differential expression.
The only two DEGs with a more than two-fold change in expression (as found with multiple microarray platforms and confirmed by qPCR) were: Plac9 (up) and Gabra2 (down).
We used a microarray platform of 60mer MUC1 glycopeptides, to confirm the presence of autoantibodies to cancer associated glycoforms of MUC1 in a proportion of early breast cancer patients (54/198).
Validation of the expression of these miRNAs in the CNS by multiplex real-time PCR confirmed the reliability of our microarray platform.
Our experiment therefore confirms that the NeuroStem Chip microarray platform can still identify gene expression changes related to early stages of differentiation of hESC into dopaminergic neurons.
We confirmed that GSEA, using this enhanced microarray platform, is able to recover results consistent with a previous analysis of fathead minnow exposure to methylmercury using standard analytical approaches.
Pairwise comparisons of replicate experiments showed correlation coefficients with r > 0.99 and were always significant (p-value < 0.01) (see Table 2), confirming a good reproducibility of the microarray platform.
18 independent experiments were used to evaluate the hybridization quality, precision, specificity and accuracy and all results confirmed the high technical quality of our microarray platform.
In general, RT-qPCR results confirmed the regulation trend seen in the microarray platform for all the 11 genes analyzed with very similar values using either GAPDH or ACTB for normalization.
