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Finally, inhibitor-treated samples were studied using a 17 K gene expression microarray platform, whereas siRNA-treated samples were hybridized on a 44 K microarray platform causing slight differences in the gene content of these two microarray platforms.
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Highly variable microarray experimental conditions and the use of different microarray platforms cause poor reproducibility of microarray measurements within and between laboratories [7] [11].
Variation in microarray-based, single cell expression measurement is contributed to by (i) technical noise intrinsic to the microarray platform; (ii) sampling effects caused by non-representative amplification of low abundance mRNA transcripts; and (iii) real differences in gene expression levels between two samples.
Different microarray platform types have been used for CSH.
This might be caused by different backgrounds in respects of ethnicity or microarray platform.
There are two major microarray platforms that have been widely used: cDNA microarrays and oligonucleotide microarrays.
We used the Affymetrix DNA microarray platform to measure the expression level of all genes and access the global effect caused by the INO-level under nutrient-limited conditions (C-limited and N-lim).
Then select the microarray platform and specific experiment to search against and the display format.
The accumulated transcribed sequences could be directly used to develop microarray platform.
The design, construction and application of a Pinus microarray platform are described.
After entering a list of genes or microarray element IDs into the ROAD, the user can then select the microarray platform and specific experiment to search against (Figure1a).
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