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Since the primary goal of this study was to improve the diagnosis of these urogenital defects and rapidly translate the findings to the clinic, DNA was analyzed using an established CGH microarray platform available for clinical diagnosis (chromosome microarray assay, CMA) [19], [20].
While microarray-based transcriptional analysis is a useful tool for functional genomics in fish, to date there has been no microarray platform available for catfish species.
In this study we have demonstrated that the microarray platform available for A. palmata can be successfully used to study evolution of scleractinian coral species of both the complex and robust clade.
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The Illumina Infinium HumanMethylation450k microarray is one of the most comprehensive microarray platforms available for the study of genome-wide DNA methylation in humans.
There are numerous microarray platforms available and they have been applied to a wide range of studies in plant biology, reviewed by Galbraith [ 17].
Although there are gene expression microarray platforms available for various species, most are based on somatic cell expression and it has been shown that the embryonic transcriptome differs significantly from that of somatic cells [ 13].
As the number of microarray platforms available for non-traditional model species is continually increasing [axolotl: [ 39], dolphin: [ 40], butterfly: [ 41, 42], coral: [ 43], many fish species, for review: [ 44], crustaceans: [ 45- 47]], researchers focusing on these and related model systems will continue to benefit from the relatively low cost of array hybridizations.
The design of this microarray (platform) is available from Gene Expression Omnibus [ 31] under accession number GPL7150.
A map and gene list for the described microarray platform is available at Gene Expression Omnibus website, accession number GPL1930.
The aCGH technique is strongly applicable to the growing number of non-model species groups for which a single microarray platform is available.
The microarray platform, freely available through NimbleGen Systems, provides a robust, cost-effective approach for genome-wide expression profiling in P. tetraurelia.
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