Then select the microarray platform and specific experiment to search against and the display format.
After entering a list of genes or microarray element IDs into the ROAD, the user can then select the microarray platform and specific experiment to search against (Figure1a).
The objective of this study was to investigate radiation-induced late changes in cutaneous gene expression using a microarray platform and quantitative, real-time, reverse-transcriptase polymerase chain reaction (RT-PCR) validation.
We previously developed a custom miRNA microarray platform, and here we describe the protocol we used to work with it including the oligo design strategy, the microarray printing protocol, the target-probe hybridization and the signal detection.
All GEPs were performed using the Affymetrix microarray platform and standard procedures.
Their importance lies in the fact that they were similar in all bladder cancer types, regardless of population, sampling method or microarray platform and experimentation procedure.
We developed an mRNA expression-based model that can predict prognosis/outcomes of human breast cancer patients regardless of microarray platform and patient group.
Table 2 gives an overview of the 16 studied data sets with information on outcome, microarray platform and number of included samples and genes.
We would like to thank the staff of the Genome Quebec Microarray Platform and the technical staff for expert help and microarray processing.
The percentage of overlapping microRNAs between each microarray platform and qRT-PCR was a measure of the reproducibility of lists of differentially expressed microRNAs.
This may be due to technical differences such as concentration of interferons, microarray platform and gene annotation, but may also reflect cell-specific (professional immune vs non-immune) responses to IFN stimulation [54].
