Sentence examples for microarray performance the from inspiring English sources

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Microarray analysis: The extracted RNA was processed for microarray performance; the platform used for that purpose was C. elegans (V2) Gene Expression Microarray 4×44K (Agilent technologies), following manufacturer instructions.

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In this paper, we examined the effect of sample integrity on microarray performance through the use of samples with a progressive decrease in RNA quality that was indexed using a sensitive automated metric of RNA degradation (RIN).

Although the problem of cross-hybridization (i.e. false-positive hits) that was observed with the 70-mer oligonucleotide arrays did not become apparent with the Affymetrix arrays, we could clearly observe an improved microarray performance with the Affymetrix arrays.

Microarray performance depends upon the ability to screen samples against a vast array of probes with the appropriate sensitivity and selectivity.

Next, the effect of the vRNA-depletion on the microarray performance was studied.

Although we could indeed see some improvement of the microarray performance after RNase H digestion (data not shown), the improvement was less pronounced compared to direct vRNA removal using the GlobinClear system.

The Agilent RNA spike-in control targets are a set of 10 in vitro-synthesized poly-adenylated transcripts derived from the adenovirus E1A gene used to monitor the labeling reactions and the microarray performance.

CK, MP and PW assisted in the microarray performance and analyses.

The quality of the hybridization and overall microarray performance was determined by visual inspection of the raw scanned data to exclude artifacts, scratches and bubbles.

Potential batch effects for culture date, RNA processing data and microarray performance date were corrected during the analysis, with no major effect on the analysis.

The main purpose of the bacterial spikes added before the hybridization step is to control the consistency of hybridization conditions across all samples, assessing the overall microarray performance [ 82].

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