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The Mouse Exon 1.0 ST array protocols include cDNA generation with random primers and cannot distinguish between transcripts with and without polyA tails, which results in a common microarray pattern for polyadenylation at an upstream site and cleavage that produces a protected 5'-fragment without a polyA tail.
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Where patterns of expression do have similarity between apple and tomato it is probable that the microarray pattern of expression represents the actual pattern of expression for those genes, since the expression pattern has effectively been confirmed in another species.
A unique microarray pattern was obtained for each Eucalyptus tree.
In this context, direct comparison of signal intensity profiles suggest that the cDNA chip developed will allow the genome-wide fingerprinting of the seven E. grandis genomes since a unique microarray pattern was obtained for each individual tree.
For the remaining six enzymes the qRT-PCR pattern differed from the microarray pattern possibly because the RT-PCR primers were amplifying different alleles or genes than those detected by the microarray oligo.
Of the top 23 ranked genes in S. cerevisiae (according to our model), 19 had an expected microarray expression pattern for Met4 regulation.
Real-time PCR was performed to verify the microarray expression pattern for a few selected OsARM genes, expressed significantly during three abiotic stresses and in various rice tissues/developmental stages.
MC designed the microarray patterns.
The data points showed a more scattered pattern for microarrays than for the EST data; however, this difference was likely caused by the smaller number of tissue types represented in the EST data.
MiR-589 was identified from the microarray study with up-regulation pattern for cases over controls in CA group (Fold-Change = 6.9, p = 0.013).
Because we wanted to compare cDNA microarray gene expression patterns for all populations in a nested ANOVA design (see below), the use of a common reference mRNA to be competitively hybridized to treatment mRNAs coming from the different populations seemed to be more appropriate for statistical analysis than a design involving the correct pairing of all nine samples [ 69].
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