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By contrast, Q-PCR analysis confirmed the microarray outcome for what concern the transcriptomic pattern of the metallothionein mt10 gene.
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Sequencing and microarray outcomes from multiple experiments suggest that our simulator will be useful for guiding NG transcriptome sequencing projects in a wide range of organisms.
While long-term survival data are not yet available for the specific patients whose tumors are included in the COG microarray, the outcome for neuroblastoma patients can be reasonably predicted on the basis of well-characterized criteria that include histology, amplification status of the MYCN gene, age, and stage [28].
Integrating interactome data thus improves classification of cancer outcome for the investigated microarray technologies and cancer types.
Kaplan-Meier plot analysis of Affymetrix microarray datasets similarly showed that high A3B expression is associated with poor outcome for ER+, but not ER−, breast cancer.
Successful application of our model to predict the clinical outcome for cetuximab in a cohort of mCRC patients using a PAS profile derived from microarray gene expression data represents a true validation of the predictive strength of the OncoFinder PAS algorithm.
Feature selection and classifier selection are two core steps in gene expression microarray-based clinical outcome classification for disease.
Our method incorporates multivariate correlations in microarray data for survival outcomes.
This is one of the largest collections of breast cancer samples (n = 118) analyzed by microarray and for which the clinical outcome is known.
Multiple reasons have been proposed to explain this lack of reproducibility in the GEP studies on CRC, such as underpowered studies, lack of validation of results, differences in experimental protocol and statistical pitfalls in analysing microarray expression data for cancer outcome [3].
DNA microarrays have already been widely used for the classification of tumor subtypes or clinical outcomes for the diagnosis, treatment, or prognosis of cancer for many years [ 1- 6].
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