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Those studies relied on mostly indirect evidences using microarray or real-time RT-PCR.
miRNA expression is commonly measured by microarray or real-time polymerase chain reaction (RT-PCR).
This is similar to the correlation observed in comparison of transcription data for 1375 human genes using microarray or real-time PCR analysis [ 65].
The genes included in the analyses were shown to be differentially expressed (microarray or real-time RT-PCR) between F and L, G+ and G- or both.
Good RNA quality assessment is considered one of the most critical elements to obtain meaningful gene expression data via microarray or real-time PCR experiments.
Two ambitious adjuvant clinical trials in breast cancer aim to prospectively assess the utility of gene expression prognostic signatures through microarray or real-time PCR analyses and will compare these methods to conventional prognostic strategies.
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This "interferon signature" can be identified by using microarrays or real-time PCR.
To evaluate transcript variations carefully, it is common to use stable housekeeping genes (HKG) to normalise quantitative microarrays or real-time polymerase chain reaction results.
Transcript levels of genes in RNAi-mediated depletion backgrounds and msl gene mutants have also been examined in diverse cell lines, embryos, and larvae using hybridization of transcript populations to gene expression microarrays or Real-time PCR [ 20, 23- 25].
Values shown are ratios of the means of three independent measurements from microarray data or real-time RT-PCR data.
The next two columns display fold enrichment of ChIP sample over input detected by microarray hybridization or real-time PCR, respectively.
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