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The eFP Browser software is easily adaptable to microarray or other large-scale data sets from any organism and thus should prove useful to a wide community for visualizing and interpreting these data sets for hypothesis generation.
We hope that many researchers will be able to use the eFP Browser both to understand particular microarray or other experimental results, as well as to communicate their own findings.
The conciseness and powerfulness, together with the applicability to data with relatively low SNP density, are convenient for analyses of genotype data produced by DNA microarray or other technologies.
The mRNA level of this target gene can be measured experimentally using a GFP conjugated to GAL1, which can be measured for intact cells, or by means of a microarray or other assay that requires lysing a small fraction of the cells under observation.
Such inappropriately expressed genes may be detected by microarray or other expression analyses of tumour against normal tissue.
As our procedure is automated, when new information and microarray or other data become available, the entire procedure can be readily repeated.
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However, there is often a paucity of suitable brain-region specific expression data obtained via microarrays or other high throughput means.
Microarrays or other expression profiling methods yield sets of differentially expressed genes that can be used to interrogate pathway databases and identify altered processes/pathways (36– 40).
Given differences in the experiments, we used Distance Weighted Discrimination (DWD) [ 17] to minimize systematic microarray data biases attributable to RNA source, different analysis laboratories, different microarrays, or other systematic differences which include sex and strain in this case.
The characterization of the transcripts captured in the library and the selection of non-redundant clones will certainly aid the annotation of genomic sequences [ 30] and the construction of microarrays or other tools for functional genomics [ 33].
The list of ToxArray™ genes was compiled from our own data, as well as extensive information from published studies investigating gene expression changes, assessed using microarrays or other technologies, following toxicant exposures.
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