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Microarrays can now be designed using either cDNA microarray or oligo-based platforms using the EST information.
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The cDNA or oligo-based microarrays add to the developing suite of genome analysis approaches in soybean [ 17].
There were also 10 other "resident" cDNA- or oligo-based platforms that were manufactured at and used by the individual centers.
The melon microarray is an oligo-based (60-mer) microarray representing a total of 17,443 unigenes derived from 33,418 high-quality melon ESTs [ 29].
To further gain information about function to LsRXR we conducted a microarray experiment using a 44K oligo-based microarray where we compared lice injected with dsRNA from LsRXR (the two different fragments) and lice injected with dsRNA from a control gen (cod trypsin).
After 2005, high background cDNA microarrays were generally replaced by oligo-based microarrays generated by companies such as Affymetrix and Agilent.
The measured magnitude of regulation was rather small for the majority of these genes (<∼2-fold), perhaps due to the incomplete synchronization and variability inherent in the starvation method, or the limited sensitivity of oligo-based microarray detection.
Gene expression was determined using U133-X3P Human GeneChip oligo-based microarray (Affymetrix, Santa Clara, CA, USA) and the corresponding microarray dataset was publicly available at the ArrayExpress web site (http://ebi.ac.uk/arrayexpress/) under accession number E-GEOD-7377 E-GEOD-7377 E-GEOD-7377
Gene expression was determined using the U133-X3P Human GeneChip oligo-based microarray (Affymetrix, Santa Clara, CA, USA), and the corresponding microarray dataset was publicly available at the ArrayExpress website (http://ebi.ac.uk/arrayexpress/) under accession number E-GEOD-11395.
cDNA labelling and microarray hybridization and was done exactly as previously described [7], using oligo-based microarrays (TIGR).
Oligo-based microarray experiments on Arabidopsis [37] allowed the detection of circadian oscillations in mRNA abundance of 5 6% of the 8200 genes examined.
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