Sentence examples for microarray observations were from inspiring English sources
The microarray observations were validated by quantitative reverse transcription-PCR (qRT-PCR) for some representative transcripts.
The microarray observations were validated by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) for several representative transcripts (Table 1).
For microarray analysis, four observations were analysed per sample, as on each array each cZIP probe was spotted in quadruplicate.
Outliers were identified based on the range across the five replicate probes per microarray as observations being >2.0 (on the log scale), when the difference between the maximum and/or minimum value and the median value was >0.95.
Their hidden state was the occupation of nucleosome, and the observations were microarray Cy3 intensities.
These observations were confirmed by microarray analyses showing that the gene expression profile characteristic of hESCs (high Oct-4, Nanog, Sox2, Cripto and Nodal, etc).
Several key observations were made from the examination of the four miRNA microarray data domains.
The microarray-based observation was further supported by RT-PCR measurements of AR expression in tumors derived from the VCaP cells implanted in mice (P < 0.001; Figure 3B), in sharp contrast to siRNA-treated cells where AR mRNA level was significantly reduced (P = 3.49e-5; Figure 3C).
Reviewer 3: 1) The text reads that "these observations are in exact agreement with microarray transcriptional profiles".
These observations are supported by an earlier report that, using microarray methodology, also identified p53 BP2 (ASPP2) downregulation in breast cancer [ 33].
