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There are other, more comprehensive cDNA microarray methods available, which produce copious amounts of data.
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To have accurate results we have chosen two different methods for GYPB*S/GYPB*s genotyping: the allele-specific AS-PCR, an "in house" method, and the HEA (i.e. Human Erythrocyte Antigen) BeadChip, a microarray method commercially available.
Statistical analysis on microarray data was performed using the significance analysis of microarrays (SAM) method, available on the World Wide Web.
The NM-CGH microarray method is currently available for mutation analysis in our laboratory.
The data were analyzed using the statistical methods available in the GeneSifter microarray data analysis system.
Detailed DNA microarray methods and microarray data associated with this publication are available through the Gene Expression Omnibus [GEO GSE29801].
The PPLR (probability of positive log-ratio) method uses information about probe level variability [ 12] Clearly there are many methods available for analyzing data resulting from microarray experiments.
Summary: The current methods available to detect chromosomal abnormalities from DNA microarray expression data are cumbersome and inflexible.
It is not easy to write a critical review of the methods available for labeling RNA and DNA "extracts" for microarray studies.
There are several methods available for pre-processing data profiled on Affymetrix microarrays [ 1, 34].
While there are several reliable amplification methods available, most amplify an antisense RNA which is suitable for cDNA microarray analysis.
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