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New automated analytical platforms based on FISH technologies and tissue microarray methods are providing a rapid means to determine the impact of consistent genomic aberrations in clinical trials, and in studies designed to investigate differential chemotherapeutic response.
Expanded microarray methods are provided in the Detailed Materials and Methods (Appendix S1).
Commercial DNA microarray methods are marketed and increase the convenience of those tests [ 97].
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Microarray methods were modified from [ 92].
It is important to note that in our hands the reproducibility of both the qRT-PCR and oligonucleotide microarray methods is very high [ 22, 23].
ERα status associated genes identified in previous breast cancer studies [ 12, 13] using DNA microarray methods were compared with our SAGE findings.
In the study by Bermúdez et al. [ 14], microarray methods were used to examine the expression of linearly amplified RNA from individual oocytes and groups of five oocytes.
For these reasons, it would appear that the possible biases arising from the microarray method are better controlled and mitigated [ 9] in current experiments than are the biases that arise from using the abundance within the EST database to estimate expression levels [ 21].
All the mutations detected by the microarray method were also confirmed by conventional DNA sequencing.
The NM-CGH microarray method is currently available for mutation analysis in our laboratory.
The DNA microarray method is found to be more sensitive in comparison to labor-intensive semiquantitative RT-PCR method, which gives data for a few gene transcripts.
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