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The results of this study show that the microarray method described is suitable for preliminary diagnoses or for confirmation of the main potential pathogens in aquatic animals.
Three separate labelling reactions were carried out on each RNA sample, giving three arrays for each time point using the microarray method described previously [ 16].
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Nuclei were then stained with bisbenzimide (5 μM) for 30 min. Gene expression was measured in duplicate samples using Real time PCR and nucleotide sequences obtained from TaqMan (identical to those used in the microarrays) using the method described above.
The microarray experiments were conducted according to the method described earlier (Kittaka et al, 2008).
Microarray experiments were conducted according to the method described previously (Noda et al, 2009).
Microarray data was analyzed by a statistical method described previously with slight modifications [ 26].
The method described above produced 737 transcripts that matched the microarray probes (E-value < 1).
The custom microarrays were manufactured by NimbleGen Systems, Inc. using the maskless photolithography method described previously [ 77].
Construction of cDNA microarrays, synthesis of probes and microarray hybridization were performed in methods described previously [ 22– 22].
Microarray slides were constructed according to methods described previously [26].
A comparable alternative to the methods described here are microarrays, which can also provide broad range detection.
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