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The PS microarray method also achieved high rates of accuracy on single sperm.
The protein microarray method also consumed far less samples than ci-ELISA.
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All the mutations detected by the microarray method were also confirmed by conventional DNA sequencing.
High-throughput microarray methods have also been developed to probe RNA structures (33− 33−.
While these results demonstrate considerable consistency between induction measured by short-read sequencing of oligo dT) primed, double-stranded cDNA and induction measured by traditional microarray methods, they also suggest that higher depth of coverage may be needed to accurately assay weakly induced genes.
The microarray (DNA chip) method also resulted in a significant number of false negative results.
Validation of the microarray method by qPCR was also performed by direct comparison of fold-changes obtained by the two methods only in the subset of samples analyzed by microarrays (n = 3 4 per group).
Each method has been demonstrated to produce array data in which an increased number of non-globin gene transcripts are detected [ 1, 10], and Debey et al[ 11] have shown that another globin RNA reduction method also improves microarray performance with whole blood samples.
The ratio magnification was also observed when the ADGE microarray method was applied to the whole microarray chip.
Comparative analysis with cultivated rice cDNA sequences has also been developed using the microarray method.
The method also provides a platform-independent view of important statistical properties of microarray data.
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