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To ensure that the microarray measurements from the HaCaT samples and the UMSCC1/1CC8 cell lines are comparable, we normalize all microarray measurements using fRMA [ 41].
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Accession I microarrays measurements used five-week old plants grown under short day conditions (10 14 hours light:dark) [31], while Accessions II analyzed RNA from 12-day old plants grown under long days (16:8 hours light:dark) [32].
For the purposes of this report, only the microarray measurements generated using Illumina BeadArrays will be used for all data analysis and results unless otherwise specifically indicated.
Instead of using existing microarray measurements, we use existing information about the operons' structure to reduce the noise in microarray data.
Spearman's Rank Correlation was used to compare microarray and RT-qPCR measurements using the SigmaStat 3.5 software (Systat Software Inc., San Jose, California, USA).
Three biological replicates for each of the two subunit cell lines (RIα and RIIβ) and the parental OVCAR cells were used for microarray measurement analysis using several different platforms including Illumina, Agilent, and Affymetrix [ 30].
Initially the simulated 'normal' and 'disease' panels were used to assess the accuracy of fold change measurements using microarrays.
The electrochemical properties of the SnO2 films with lithium were studied by in situ conductivity measurements using an interdigitated microarray electrode as well as by cyclic voltammetry, galvanostatic cycling measurements and ac-impedance spectroscopy in 1 M LiClO4/ PC+EC).
This was evaluated using microarray data [4] or by direct measurements using RPA.
For each single-cell measurement, a training set of single-cell amplification microarray measurements was used to normalize probe intensities across each chromosome.
At least 4 statistically independent microarray measurements were used to characterize each physiological situation; with the exception of the experiment where thyroid hormone actions were studied where RNA pooled from 5 different mice was used.
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