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Technical validation of microarray measurements of gene expression was done with qRT-PCR and has been reported [34].
When microarray measurements of differential expression are compared to qPCR, it is well known that microarrays provide a smaller dynamic range [3].
The discrepancy between qPCR and microarray measurements of IL-1β transcript levels was likely to be due to high signal values observed in resting monocytes for the 2 IL1B probe sets in microarray analysis (Figure 4).
To date, the main sources of such data are microarray measurements of genome-wide expression profiles, with over 400,000 profiles stored in GEO [1] alone as of April 2010.
This resulted in a dataset of 72 microarray measurements of 25,280 genes.
The PCs obtained when PCA was applied to the Sty microarray measurements of genotype are shown in Figure 4.
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The three 2004 Expression Analysis Pilot Proficiency Test collaborative studies, each with 13 to 16 participants, provide triplicate microarray measurements on each of two reference RNA pools.
A set of microarray measurements on PCa tissue samples used for validation was downloaded from GEO accession GSE8218 [ 35, 36].
A previous 4C assay, based on microarray measurement of amplified 3C products, suggested that interchromosomal interactions did not contribute significantly to the nuclear microenvironment of the β-globin locus [23].
Our measurement of the large-scale organization of gene expression levels is associated with biological differences between the brains of animals separated by behavior or by postnatal experience, nonetheless, it is possible that a biological property of the brains influences the microarray measurement of gene expression levels but does not influence actual gene expression levels.
Microarray measurement of ANXA10 mRNA expression was successfully validated by RT qPCR (Pearson correlation: 0.9; data not shown).
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