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DK contributed to probe design and microarray manufacturing, and performed first bioinformatical analyses.
H.H., L.N. a L.P. set and kept BASE database, optimized methods for microarray manufacturing and prepared all microarrays used in this study.
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The precise probe list and microarray manufacturing have been described previously [ 30] and customization for the current article is described in the Additional file 1.
The probe design parameters and microarray manufacturing methods were experimentally determined through hybridization experiments with a microarray of 3,900 oligonucleotide probes specifically designed for optimization tests (for details see Additional file 1, Additional file 2: Figure S1 and Additional file 3: Figure S2).
The classes microarray manufacturing, microarray washing, microarray image acquisition and microarray gene expression profiling represent different subprocesses of a DNA microarray experiment.
TR contributed to the gene list, probe and overall microarray design, and performed cloning and preparations of probes for microarray manufacturing.
Authors would like to thank Indrek Valvas and Asper Biotech for microarray manufacturing.
Probes showing the best combination of thermodynamic criteria and strain coverage were selected for microarray manufacturing (Fig. 1f).
To construct the different cDNA libraries for microarray manufacturing, P. pinaster samples of compression (Cx), opposite (Ox), early (Ex) and late (Lx) developing xylem were collected from different genotypes of a Corsican clonal population planted in 1986 in the forestry station of INRA-Pierroton (Aquitaine, France).
The class microarray manufacturing represents the process of manufacturing a microarray chip with probes of interest (relationship has participant ).
The class probe represents fragments of DNA used in the microarray manufacturing process.
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