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Ozone readings within the microarray laboratory were similar to the environmental levels.
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Clinical samples from the DeRisi laboratory were tested by PCR to confirm the microarray results.
After mRNA extraction, a custom microarray, created in our laboratory, was used to evaluate fold changes of 376 genes, including the temporal expression of decorin.
A P. pelagicus cDNA microarray developed in our laboratory was used to identify genes involved in the hardening of the crustacean exoskeleton during the molting process, and to trace their expression profiles across the entire molt cycle.
Our groups previously published a report that concluded that microarray experiments in our laboratories were excellently comparable [ 7].
Additional variability in the treatment and handling of the models and in the RNA extraction typically occur outside the microarray laboratory, and can be reduced (but not eliminated) by careful experimental design.
"The things that we're doing in the laboratory are quite advanced, things like working with microarrays and doing proteomic work," says Powlowski.
Although gene expression profiling using microarray technology is widely used in research environments, adoption of microarray testing in clinical laboratories is currently limited.
While reproducibility between identical RNA samples on different microarray platforms between dedicated laboratories is good [1], comparability between studies with independent samples is less satisfactory [2], [3].
The second constraint is that the microarray laboratory can concurrently process 6 to 8 tissue samples on DNA arrays.
Due to the fact that in our DNA-microarray laboratory validation experiments are only performed when necessary (i.e. to introduce a new scientist (experimenter) in the laboratory) confounding of some factors could not be avoided.
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