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Maize oligonucleotide array 46 K version slides were obtained from the microarray laboratory of the Maize Oligonucleotide Array Project at the University of Arizona.
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On this paper we study the process and the needs of a microarray laboratory and define its workflow.
To validate the SIGLa capability of adapting to multiples laboratories, on this paper we study theprocess and the needs of a microarray laboratory and define its workflow.
To validate the SIGLa capability of adapting to multiples laboratories, on this paper we study the process and the needs of a microarray laboratory and define its workflow.
We thank other microarray laboratory members for advice and discussions.
The PFSANGER Genechip® was purchased from Affymetrix, Inc. Array hybridization was performed at the microarray facility of the Laboratory of Immunopathogenesis and Bioinformatics, SAIC-Frederick, Inc Frederickk, MD).
The pre-publication history for this paper can be accessed here: http://www.biomedcentral.com/1755-8794/6/49/prepub We are grateful to the Laboratory of Microarray Analysis of Center of Excellence BioExploratorium (Department of Biology Warsaw University and Institute of Biochemistry and Biophysics PAS, Warsaw, Poland) for possibility of using the Affymetrix microarray platform.
Hybridization was carried out at the DNA microarrays and DNA sequencing Laboratory of the National Institute for Biotechnology in the Negev, Beer-Sheva, Israel, affiliated to our university.
The samples were hybridized using oligonucleotide DNA microarrays obtained from the laboratory of Karl Hoffmann at the University of Cambridge, UK.
Printed 70-mer oligonucleotide microarrays were obtained from the laboratory of Prof. David Galbraith at the University of Arizona.
Maize unigene-microarrays were provided by the laboratory of Prof. Schnable (Iowa University, USA) and contained 11,827 maize ESTs [ 27].
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