The gene coding for the peptidyl-prolyl cis-trans isomerase A (PPIA) was used as the endogenous control for normalization because, in the microarray data, it showed in all conditions the steadiest expression in our experimental setting when compared with other housekeeping genes.
IBSP has also been identified as a potential AC marker gene in a rat microarray study where it showed higher expression levels in AC cells than NP cells [ 23] but was not further characterised.
Using a 1200-gene mitroarray, it was shown that only three to seven genes overlapped among any two metal treatments [ 22].
Out of the 150 chicken microRNAs included in this microarray, it was shown that 15, 36, 31, 24, 15, and 11 microRNAs were differentially expressed when comparing spleen and ileum, liver and ileum, liver and cecal tonsil, liver and spleen, spleen and cecal tonsil, and cecal tonsil and ileum (P < 0.05).
dapB was selected as reference gene based on the microarray result, as it shows similar expression in all strains.
Using DNA microarrays it was shown that the lack of potassium affects the expression of more than one thousand genes including a strong activation of genes involved in sulfur metabolism, oxidative stress response and the retrograde pathway and a reduced expression of genes involved in ribosome biogenesis and cell cycle control.
The integrated analysis for mRNA microarray and miRNA microarray showed the effects of specifically inhibiting and/or enhancing the function of miRNAs on the gene transcription level.
Our data shows that microarray data can be very consistent at the same time as it shows a bias.
Although Jund is not differentially expressed between WKY and Lewis mesangial cells, our microarray analysis showed that it was markedly overexpressed in the basal (without NTN induction) and nephritic WKY glomeruli 10 days after nephrotoxic serum (NTS) injection.
The relative transcript level of each gene was normalized to nmb0956 (sucB), on the basis of the microarray data which showed it to be invariant between all conditions tested.
