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Indeed, as only exponential phase GAS was analyzed by tiling microarray it is possible that additional sRNAs would be discovered in GAS grown to other growth phases.
In ordinary mRNA expression microarray, it is a standard data processing procedure to normalize the microarray data with an assumption that the whole mRNA expression signal is constant among the samples.
If the test is based on a DNA microarray it is unlikely to be affected by the time since diagnosis and so this item will be scored as yes.
Although the increased expression of IL-6 and TNF-α is less dramatic when measured using the microarray, it is significant using Q-PCR [ 10].
If probes with similar sequences are used in the same microarray, it is possible to detect expressed genes with two different probes as cross hybridization.
For each strain i, given the set of probes representing a C58 replicon a on the microarray, it is straightforward to compute a likelihood function on the basis of this modeling.
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To prepare the antibody microarray, it was first blocked with a blocking solution for 30 min at room temperature, rinsed with Milli-Q grade water for three minutes, and then dried by centrifugation.
By microarray, it was also found to be upregulated in immortalized ovarian surface epithelial (IOSE) cells following TGF-β treatment.
Using a 1200-gene mitroarray, it was shown that only three to seven genes overlapped among any two metal treatments [ 22].
To prepare the antibody microarray, it was blocked for 30 min, then dried and the array was incubated with the biotin-labeled cell lysates at 4 °C.
Only for RAP1A, which had a very low expression level on microarray, it was not possible to observe the differential expression by RT-PCR.
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