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The test set samples were from a different experiment and have a nearly 10-fold difference in overall microarray intensity compared with the training data.
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Furthermore, transcript abundance (microarray intensity) was compared between contigs and singletons using Welch's t-test.
In terms of microarray intensity data the method was applied to compare individual differences based on ANOVA model parameters between all pairs of bacterial groups under a global error model.
Values in the table are the Spearman rank correlation coefficients comparing the averaged RNA-seq FPKM and microarray intensity values.
To assess how the amount of input RNA affects microarray results, we compared intensities found using 5 versus 100 ng of murine liver RNA (Experiment 2).
Genetic mapping in grapevine using SNP microarray intensity values.
The median microarray intensity for all microarrays was normalized to 100, and probe sets with median intensities >100 were scored.
Raw microarray intensity values are pre-processed using the Robust Microarray Average (RMA) method [ 22].
Binding events detected in addition to the marker candidates that were expected from macro-membrane screens (in analogy to SEREX screens) indicate greater detection limit/signal intensity of the microarray when compared to the membrane method.
Following the same computational strategy and using the same scaling parameters derived from brain 2 above, we scaled microarray intensities from these cell lines, and compared both measures of microarray gene expression to TPM values based on RNA-Seq.
RNA-Seq identified 3458 genes differentially expressed (p < 0.05, Bonferroni corrected), compared with 2144 identified using microarray intensities, of which 1121 agree between methods (p ~ 0).
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