PamChips were loaded into the Olympus FD10 microarray instrument according to the manufacturer's instructions.
Using a tissue microarray instrument (Beecher Instruments, Alphelys™), we removed representative areas of the tumor from paraffin embedded tissue blocks.
A fully automated tissue microarray instrument (Beecher Instruments) was used to acquire tissue cores from the donor block from our patient's cohort.
TMA construction was performed by using a special tissue microarray instrument (Beecher Instruments, New Jersey, USA), according to standard protocols [ 12, 13].
In this study we used the flow-through arrays in conjunction with the Olympus FD10 microarray instrument, which automates the required hybridization, pumping and image capture.
Four representative 0.8 mm cores of tumour areas were obtained for each patient using a tissue microarray instrument (Beecher Instruments, Silver Spring, MD, USA), as described (Kononen et al, 1998; Kallioniemi et al, 2001; Torhorst et al, 2001).
Two tissue cores (0.6 mm in diameter) were taken from each tumour sample and placed in a new recipient paraffin block using a commercially available microarray instrument (Beecher Instruments, USA).
Three 1.0-mm-diameter punches taken from each sample were mounted on recipient paraffin block with a semiautomatic tissue microarray instrument (TMA) (Beecher Instruments, Silver Spring, MD, USA) as described [ 22].
Three 1.0-mm-diameter punches taken from each sample were mounted on recipient paraffin block with a semiautomatic tissue microarray instrument (Beecher Instruments, Silver Spring, MD, USA) as described [ 23].
Using a tissue microarray instrument (Beecher Instruments, Sun Prairie, Wisconsin, USA), three representative areas of each tumor (1.0 mm diameter) were removed from paraffin-embedded tissue blocks which had been prepared at the time of resection.
