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The paired microarray information was incorporated into EDGE, using the 'MATCHED DESIGN SETTINGS' option.
All reporting and depositing of microarray information was conducted per the guidelines of the Minimum Information About a Microarray Experiment (MIAME) [ 15].
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All 501 internal patients and their clinical, pathological and microarray information are tabulated in Table S1 in Additional file 1.
For some parameters evaluated in tissue microarrays, information was not available in all patients.
The microarray image information was converted into spot intensity values using Scanner Control Software Rev. 7.0 (Agilent Technologies, Santa Clara, CA).
The microarray image information was converted into spot intensity values using Scanner Control Software Rev. 7.0 (Agilent).
Biological information, such as microarray data, was not used at all for sequence extraction, and it becomes useful only during interpretation of the extracted sequence.
High-density oligonucleotide microarray probe mapping information was provided by Ensembl except in the case of Saccharomyces cerevisiae and A. thaliana where high gene density causes erroneous mapping output in Ensembl.
Incorporation of chromosome arm-specific microarray hybridization information is providing an important cross-reference for the positioning of genes in this framework.
However, the major barrier against using genomic approaches to improve Ae. variabilis is that the genome sequence, cDNA libraries, EST databases, and microarray platform information are not available [ 14].
Generally, microarray data is information-rich, with multiple dimensions of variability.
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