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As a consequence, in microarray hybridizations using this amplified RNA, these motifs are abundantly present.
To assess differential gene expression in newborn mouse Pax6 heterozygous lenses compared to wild type lenses, we performed DNA microarray hybridizations using the Affymetrix Mouse Genome 430 2.0 Arrays.
Affymetrix microarray hybridizations using the Maize GeneChip were performed for RNA samples from three biological replicate samples for each genotype.
Affymetrix microarray hybridizations using the Ath1 whole genome array, containing probe sets representing ~22,800 genes, were performed using three biological replicate samples for each genotype.
Specificity of BAY 87-2243 for the suppression of HIF-1-mediated gene transcription on a genome-wide scale was evaluated by microarray hybridizations using Affymetrix whole genome 133Av2 arrays.
The results from the quantitative PCR for the male and female experiments were compared against the results of our microarray hybridizations using linear regression analysis [ 59] and showed a strong correlation (R = 0.84 and R = 0.67 respectively).
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Our microarray hybridizations used a universal design and were conducted as part of Community Collaboration #17 (see Table 7; [23]) under the Songbird Neurogenomics Initiative.
Previous experimental reports of the use of genomic DNA as a reference for microarray hybridizations used genomic DNA concentrations ranging from 0.1 μg to 4 μg for M. tuberculosis [ 7].
We present a theoretical model that incorporates both kinetics of the reversible hybridization reaction and diffusional transport of the labeled strands, and analyze DNA microarray hybridization using this model.
We also carried out a genome-wide gene expression analysis in normal and cancer cells of breast and prostate by DNA microarray hybridization, using Illumina's Sentrix Human-6 Expression BeadChips.
Microarray hybridization using Affymetric Human Genome HG 133 plus 2 GeneChip and statistical analysis was performed as previously described [ 44].
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