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In the RNA samples from U251 cells used for microarray hybridizations however, HSF1 expression was induced more than 1.8 folds after NFIX siRNA treatment (nor shown).
For illustration, we here use data from an experiment done by Archer et al. (2006) where the same cell extract has been used for multiple microarray hybridizations, however, either prepared with RNeasy to remove RNA degrading enzymes, or not.
Similar(58)
However, we also performed two additional microarray hybridizations in which 50 vs 180 ATU healthy fry, and 50 vs 180 ATU M74 fry, were compared.
However, the reference RNA used in the microarray hybridizations consisted of pooled RNA from the thyroid gland of 65 individuals without known thyroid disease [ 20].
Microarray hybridizations were performed in triplicates for each strain.
Microarray hybridizations were performed on the 22K Agilent platform.
The microarray hybridizations were designed as common reference experiments.
Affymetrix GeneChip® Poplar Genome Array oligonucleotide microarrays were used for all microarray hybridizations.
cDNA microarray hybridizations.
JK performed the microarray hybridizations.
MB participated in microarray hybridizations.
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