Exact(1)
In this manuscript we analyze the relationship between the amount of mRNA and the fluorescent signal from the microarray hybridizations demonstrating that for a wide-range of mRNA concentrations the fluorescent signal is a linear function of the amount of mRNA.
Similar(59)
Comparison of gene expression by microarray hybridizations and qRT-PCR demonstrated a very high level of concordance between the datasets, which is represented by a correlation coefficient of 0.94 and a Pearson correlation of 0.97 (Fig. 2).
Transforming growth factor beta-1 (TGFβ1) was selected as a transcript of biological interest that did not demonstrate an expression difference by microarray hybridizations.
For each plasmid, we performed three transfections and three microarray hybridizations.
Microarray hybridizations were performed in triplicates for each strain.
Microarray hybridizations were performed on the 22K Agilent platform.
The microarray hybridizations were designed as common reference experiments.
Affymetrix GeneChip® Poplar Genome Array oligonucleotide microarrays were used for all microarray hybridizations.
cDNA microarray hybridizations.
JK performed the microarray hybridizations.
MB participated in microarray hybridizations.
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