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In comparison to the high number of microarray hybridizations covering different conditions, only a low number of hybridizations of short RNA to the Affymetrix Symbiosis Chip were performed resulting in only few reliable sRNA candidates.
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Independent microarray hybridizations were carried out for each sample (n = 5, per experimental group) with oligonucleotide microarrays covering 34,000 transcripts representing all known and predictive genes of the rat genome (CodeLink™ Rat Whole Genome Bioarrays, GE Healthcare), following the manufacturer's protocol.
For each plasmid, we performed three transfections and three microarray hybridizations.
The microarray hybridizations from seawater samples of PDS and PHS revealed some similarities, including the presence of the Microbacteriaceae.
Microarray hybridizations were performed in triplicates for each strain.
Microarray hybridizations were performed on the 22K Agilent platform.
The microarray hybridizations were designed as common reference experiments.
Affymetrix GeneChip® Poplar Genome Array oligonucleotide microarrays were used for all microarray hybridizations.
cDNA microarray hybridizations.
JK performed the microarray hybridizations.
MB participated in microarray hybridizations.
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