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We averaged the ratios from duplicate microarray hybridizations and used the same threshold as for HeLa cells to define the target promoters occupied by Myc in foreskin fibroblasts.
Data from the DNA microarray hybridizations and the in silico hybridizations were compiled to generate Figure 6 using Spotfire (Somerville MA) for data visualization.
We are grateful to the staff of the Finnish microarray core facility at Turku Centre of Biotechnology, especially Tiia Heinonen for performing the microarray hybridizations and scanning.
We thank Jun Yang and Brandie Fullmer at the Laboratory of Immunopathogenesis and Bioinformatics, SAIC-Frederick, Inc. for microarray hybridizations and intramural editor Brenda Rae Marshall for assistance.
EF performed microarray hybridizations and analysis.
RV: performed microarray hybridizations and scanning.
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Microarray Hybridization and Original Data Normalization.
This includes the detailed procedures for ChIP and library generation for microarray hybridization and deep sequencing.
Microarray hybridization and processing, image scanning and processing were performed as previously described [18].
The microarray hybridization and data normalization analysis were performed by CapitalBio Corp [19].
Probe synthesis, microarray hybridization and data acquisition were performed as described previously [31], [73].
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