Whereas microarray hybridization probes generate maximum signal with multiple deletions, aptamer sequences with the same deletion rate result in a 3-fold binding signal reduction compared with the same sequences synthesized for maximized sequence fidelity.
For example, the expression of SAA3 was found to be 1825-fold induced infectedted udder quarter 24 h after inoculation with E. coli 1303, whereas microarray hybridization detected a 52-fold induction.
Albert, T.J. et al. Direct selection of human genomic loci by microarray hybridization.
Labelled DNA was then genotyped using DNA microarray hybridization.
Vontas, J. et al. Transcriptional analysis of insecticide resistance in Anopheles stephensi using cross-species microarray hybridization.
Microarray hybridization was performed at 65°C for 17 h in Agilent's SureHyb hybridization chambers.
About 2 μg RNA was isolated from anthers and used for RNA labeling and microarray hybridization.
RNA isolation, cDNA synthesis, and microarray hybridization were performed according to previous protocols [45].
Microarray Hybridization and Original Data Normalization.
Affymetrix microarray hybridization signals were normalized with dChip [89].
