Sentence examples for microarray hybridization we from inspiring English sources
To verify the expression changes seen in microarray hybridization, we used real-time RT-PCR to corroborate expression levels of selected genes.
To validate gene expression patterns of microarray hybridization, we performed Q-RT-PCR as described [8] for 4 genes (Mcl-1, Bcl-xL, Tob1 and Atf3).
To confirm the miRNA composition determined by microarray hybridization, we measured the cellular and extracellular mature miRNA populations by quantitative PCR approaches after reverse-transcription of miRNAs (qRT-PCR).
After microarray hybridization, we had complete data sets on 44 arrays from 15 welders and 7 controls available for subsequent analysis.
Once again, with our cross-species microarray hybridization we were able to detect the presence of SPIN transcripts in bovine, mouse and Xenopus laevis oocytes, and this was also confirmed by RT-PCR and gene-specific cross-species microarray hybridizations.
As an alternative to the Washington Genome Center PAO1 strain that was used for microarray hybridization, we also tested PAO1 DSM1707 which like PA14 resulted in PCR products corresponding to the published sequence of PA4684/4685.
To validate the results from the microarray hybridizations, we chose several mRNAs at random from each list of 106 and used RT-PCR to confirm their interactions with the respective eIF3 complexes.
For isolation of RNA from large numbers of cultured cells, standard RNA extraction and microarrays hybridization we followed standard protocols as described elsewhere [ 6, 16].
Using microarray hybridization data, we compare the gene expression of Corynebacterium glutamicum under acetate and glucose feeding conditions: Known regulatory networks are confirmed, but moreover CoryneCenter points out additional regulatory interactions.
Based on studies of SNPs and gene contents by comparative microarray genome hybridization, we present an illustration of how different genomotypes of B. grahamii are distributed across nearby geographic locations.
On the basis of the gene-to-gene correlation coefficient calculated from 67 microarray hybridization data points, we performed a network-based analysis.
