Microarray hybridization was performed in the Roswell Park Genomics Shared Resource using the Illumina human Bead-ChIP array according to the manufacturer's instructions.
Microarray hybridization was performed at 65°C for 17 h in Agilent's SureHyb hybridization chambers.
Microarray hybridization was performed according to the Affymetrix GeneChip Expression Analysis Technical Manual (www.affymetrix.com).
Microarray hybridization was performed at the NINDS microarray consortium using zebrafish Affymetrix oligonucleotide arrays.
DNA labelling and microarray hybridization was conducted by staff at the W.M. Keck Facility at Yale.
Microarray hybridization was performed according to the Agilent Technologies protocol, with the following modifications.
Microarray hybridization was carried out in an ArrayBooster Hybridization Station (Advalytix, Brunnthal, Germany).
Microarray hybridization was carried out using Illumina's RatRef-12 Expression BeadChips arrays (22, 523 probes) (Illumina Inc., San Diego, CA).
RNA sample amplification, dye labeling, and microarray hybridization was conducted as part of Community Collaboration under the Songbird Neurogenomics Initiative and used a universal reference design [23].
The RIN of the samples used for microarray hybridization was 8.7±0.3 (mean ± SD) and it was 8.8±0.2 for the samples used in qPCR analysis.
Microarray hybridization was performed on three different ectopic thyroids (two in duplicate, one in quadruplicate) and compared to RNA of thyroid tissue from a Caucasian female, age 68 y with gall bladder cancer (Ambion, #AM6872).
