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In previous studies, we used cross-species microarray hybridization to hybridize S. macrospora targets on N. crassa cDNA or oligonucleotide microarrays [ 8- 11]; however, in these analyses, less than 50% of all genes on the arrays gave a significant signal.
To test if the isolated cDNAs are expressed in the manner expected (i.e. are induced by one or more of these polysaccharides but absent when grown on glucose) we selected unique cDNAs representing 728 contigs and hybridized them by conducting single-channel, slide-based microarray hybridization to labeled cDNAs made from fungal cultures grown on individual polysaccharides.
In the present study, we used microarray hybridization to investigate the pleiotropic role of HU in the cell by studying genome-wide gene expression as a function of the genetic hupA, hupB, hupAB and wild-type backgrounds at three different growth phases.
Song [ 58] used microarray hybridization to identify genes involved in photosynthesis and chlorophyll synthesis in response to heat stress, but no metabolic pathways were mentioned.
The expression profile of the maize IBM2 Syn10 double haploid population [ 19] was determined by microarray hybridization to 60-mer probes.
All of the microarray expression distributions were bimodal, with a lower mode that presumably corresponds to background microarray hybridization to probes matching lowly or nonexpressed genes.
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We have also performed microarray hybridizations to check its usability.
Our experimental method was to culture yeast in the presence and absence of berenil, isolate total RNA, and conduct microarray hybridizations to measure changes in steady state transcript levels for all yeast genes.
The current experimental database of Soleamold includes nineteen normalized and scaled microarray hybridizations to compare the global pattern of gene expression during ovarian and testicular development, including data from males treated with different hormones, and few ISH experiments.
The procedure for printing the microarrays, hybridization to the microarrays and analysis was previously described [ 4].
This technique has evolved from pioneering studies utilizing microarray hybridization techniques to score presence/absence of marker regions adjacent to restriction sites in the genome [23], to exploit next-generation techniques to directly sequence these regions [6].
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