Sentence examples for microarray hybridization signals from inspiring English sources
The genotypes inferred from the cDNA microarray hybridization signals of pear cultivars such as 'Lijiang Huangsuanli', 'Xiuyu', 'Midu Yuli', 'Baimianli', and 'Deshengxiang' were similar to the known genotypes of all tested cultivars.
Along with exploration in microarray technology applications, novel strategies and approaches for experimental controls and design are needed to ensure that microarray hybridization data from different samples are comparable, interpretable, and biologically significant because of the inherent variability in microarray hybridization signals.
Affymetrix microarray hybridization signals were normalized with dChip [89].
Our main results are the scatterplot showing microarray hybridization signals using a probe derived from muscle mRNA, versus the signals using only an oligo dT) probe (Fig. 2).
This approach is derived from the experimental findings of Hughes et al. that microarray hybridization signals are mostly influenced by mismatches in the solution-end (distal part) rather than surface-end (proximal part) portion of the oligonucleotide probe [ 15].
The remaining 1751 probes showed significant variability in microarray hybridization signals among E. coli O157 H7 strains (Table 3), and the ORFs that they represent were designated as variably absent or present (VAP).
Synthesis and labeling of cDNA as well as DNA microarray hybridization, signal detection and data analysis followed protocols described previously [ 23].
Real-time PCR quantification failed to amplify ESTs C06013D07 and IC0AAA56DH07 that exhibited, respectively, microarray hybridization signal ratios of 0.23 and 0.30 in ITA, indicating that microarray data of these two ESTs was most likely affected by nonspecific cross-hybridization.
The microarray hybridization signal intensity values of the 22,283 U133A probe sets were MAS5.0 calibrated, and the values were scaled by adjusting the mean signal intensity values to a target value of log500.
It was known that this probe cross-hybridizes slightly to fragments of the meningococcal gene in strain FA1090 (28 bp at 96% and 37 bp at 89%), but not at a level that would be expected to be detected on the Pan- Neisseria microarray and which did not, in fact, generate a microarray hybridization signal.
The use of base-modified analogs of nucleoside triphosphates has been shown to increase complementary duplex stability in other applications, and here we attempted to enhance microarray hybridization signal across a wide range of sequences and expression levels by incorporating these nucleotides into labeled cRNA targets.
