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Subsequent gene selections were based on microarray hybridization scores, proceeding from most abundant to least abundant.
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This technique has evolved from pioneering studies utilizing microarray hybridization techniques to score presence/absence of marker regions adjacent to restriction sites in the genome [23], to exploit next-generation techniques to directly sequence these regions [6].
Albert, T.J. et al. Direct selection of human genomic loci by microarray hybridization.
Vontas, J. et al. Transcriptional analysis of insecticide resistance in Anopheles stephensi using cross-species microarray hybridization.
Labelled DNA was then genotyped using DNA microarray hybridization.
Microarray hybridization was performed at 65°C for 17 h in Agilent's SureHyb hybridization chambers.
About 2 μg RNA was isolated from anthers and used for RNA labeling and microarray hybridization.
RNA isolation, cDNA synthesis, and microarray hybridization were performed according to previous protocols [45].
Microarray Hybridization and Original Data Normalization.
The microarray hybridization and Smith-Waterman alignments for strains ET3-1 and MSA553 were used to verify the accuracy of the in silico scoring system.
Affymetrix microarray hybridization signals were normalized with dChip [89].
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