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We thank Marja Nieuwland for mRNA isolation, labeling and microarray hybridization; members of our laboratory, Paul Koppen, Maarten Fornerod and Bernike Kalverda for helpful discussions; and Pam Geyer for providing SU HW) antibody.
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However, this could be due to the relatively low expression level of all members of the PDGF family in PTC, making them difficult to quantitate by cDNA microarray hybridization.
Albert, T.J. et al. Direct selection of human genomic loci by microarray hybridization.
Vontas, J. et al. Transcriptional analysis of insecticide resistance in Anopheles stephensi using cross-species microarray hybridization.
Labelled DNA was then genotyped using DNA microarray hybridization.
Reimer, J. J. & Turck, F. Genome-wide mapping of protein-DNA interaction by chromatin immunoprecipitation and DNA microarray hybridization (ChIP-chip).
Microarray hybridization was performed in the Roswell Park Genomics Shared Resource using the Illumina human Bead-ChIP array according to the manufacturer's instructions.
Microarray hybridization was performed at 65°C for 17 h in Agilent's SureHyb hybridization chambers.
About 2 μg RNA was isolated from anthers and used for RNA labeling and microarray hybridization.
RNA isolation, cDNA synthesis, and microarray hybridization were performed according to previous protocols [45].
Microarray Hybridization and Original Data Normalization.
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