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Incorporation of chromosome arm-specific microarray hybridization information is providing an important cross-reference for the positioning of genes in this framework.
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Expression patterns derived from microarray hybridizations provided further information on conditions of expression of a number of sRNA candidates.
Details of probe design and microarray hybridization are available in the Supporting Information.
Thus it seems that our microarray hybridization approach is capable of providing information about A. niger gene expression modulation with a considerably high level of confidence.
Real-time RT-PCR analysis of selected genes showed by our microarray hybridization approach was capable of providing information about S. cerevisiae gene expression modulation with a considerably high level of confidence.
Thus, it seems that our microarray hybridization approach is capable of providing information about S. cerevisiae gene expression modulation with a considerably high level of confidence and is an open source of data for further investigation for the mechanisms of propolis-mediated cell death in all susceptive organisms.
In addition, the OliD program displays the location of cross-hybridizing regions as this information might be useful during the microarray hybridization analysis.
Our microarray hybridization experiments performed in this study provide quantitative information on the binding affinity of individual mismatched duplexes by means of the hybridization signal intensity (fluorescence of hybridized targets).
In the attempt to standardize the bias-prone technique of microarray hybridization, the international Microarray Gene Expression Data (MGED Groupp has developed standards for the Minimal Information About a Microarray Experiment [ 21].
In contrast, Diversity Arrays Technology (DArT) is a microarray hybridization based technique that permits simultaneous screening thousands of polymorphic loci without any prior sequence information [ 34].
Albert, T.J. et al. Direct selection of human genomic loci by microarray hybridization.
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